| Fecha | Local | Visitante | Resultado |
|---|---|---|---|
| 23/05/21 15:30 | Hamburger SV | Eintracht Braunschweig | 4:0 |
| 23/05/21 15:30 | Hannover 96 | 1. FC Nürnberg | 1:2 |
| 23/05/21 15:30 | FC Würzburger Kickers | SC Paderborn 07 | 1:1 |
| 23/05/21 15:30 | Erzgebirge Aue | VfL Osnabrück | 2:1 |
| 23/05/21 15:30 | VfL Bochum | SV Sandhausen | 3:1 |
| 23/05/21 15:30 | Holstein Kiel | SV Darmstadt 98 | 2:3 |
| 23/05/21 15:30 | Jahn Regensburg | FC St. Pauli | 3:0 |
| 23/05/21 15:30 | SpVgg Greuther Fürth | Fortuna Düsseldorf | 3:2 |
| 23/05/21 15:30 | 1. FC Heidenheim 1846 | Karlsruher SC | 1:2 |